R-GNOSIS aimed at determining the efficacy and effectiveness of cutting-edge interventions to reduce carriage, infection and spread of Multi-Drug resistant Gram-negative Bacteria (MDR-GNB).

The project combined five international clinical studies, all supported by highly innovative microbiology, mathematical modelling and data management.

The five clinical studies investigated the following interventions:

• A Point-Of-Care-Testing guided management strategy to improve appropriate antibiotic prescription for uncomplicated UTI in primary care
• Gut decolonization followed by fecal microbiota transplantation in outpatients with intestinal carriage of MDR-GNB
• A “test and prescribe” strategy, based on rapid diagnostic testing of faeces for MDR-GNB to optimize antibiotic prophylaxis in colo-rectal surgery
• Contact Isolation of patients with ESBL-producing Enterobacteriaceae in general hospital wards
• Three Decolonization strategies in ICUs